Which of the Following Tools of Recombinant DNA Technology Is

Question

Which of the following tools of recombinant DNA technology is incorrectly paired with its use?
A) restriction enzyme-analysis of RFLPs
B) DNA ligase-cutting DNA, creating sticky ends of restriction fragments
C) DNA polymerase-polymerase chain reaction to amplify sections of DNA
D) reverse transcriptase-production of cDNA from mRNA
E) electrophoresis-separation of DNA fragments

Accepted Answer

DNA ligase is used to join or seal DNA fragments together, while restriction enzymes are used to cut DNA at specific sequences, producing fragments that can be analyzed or manipulated. Thus, the pairing of DNA ligase with cutting of DNA is incorrect.