Which of the Following Uses Reverse Transcriptase to Make CDNA

Question

Which of the following uses reverse transcriptase to make cDNA followed by amplification?
A) Southern blotting
B) Northern blotting
C) Western blotting
D) Eastern blotting
E) RT-PCR

Accepted Answer

RT-PCR (Reverse Transcriptase-Polymerase Chain Reaction) uses reverse transcriptase to make cDNA from RNA followed by amplification. Southern, Northern, Western, and Eastern blotting are all different techniques that involve transferring biomolecules to a solid support for detection or analysis, but do not involve reverse transcriptase or cDNA amplification.