Which PCR Step Causes the Denaturation of Double-Stranded DNA

Question

Which PCR step causes the denaturation of double-stranded DNA?
A) Add DNA polymerase and nucleotides at 72° C.
B) Cool DNA to between 50° C and 65° C.
C) Add primers.
D) Heat target DNA to 94° C.
E) Repeat the cycle of heating and cooling.

Accepted Answer

The denaturation step in PCR involves heating the target DNA to around 94° C to separate the double-stranded DNA into single strands, allowing primers to anneal in subsequent steps.