Which of the Following is Not True of ﬂUorescent in Situ

Question

Which of the following is not true of ﬂuorescent in situ hybridization (FISH)?
A) Probes can be applied to intact cells.
B) Probes can locate genes on chromosomes.
C) It can be used to identify unknown bacteria without culturing.
D) It uses electrophoresis to separate the DNA.
E) It can be used to detect RNA in cells.

Accepted Answer

Fluorescent in situ hybridization (FISH) does not use electrophoresis to separate DNA. FISH involves using fluorescent probes that bind to specific parts of the chromosome or RNA molecules within the cell, allowing for the visualization of genetic material without the need for electrophoresis.