Analysis of DNA Fragments in Gel Electrophoresis is Based on ______

Question

Analysis of DNA fragments in gel electrophoresis is based on ______.
A) larger fragments moving slowly and remaining closer to the wells
B) DNA having an overall negative charge and moving to the positive pole
C) DNA fragments being stained so that they can be seen
D) application of an electric current through the gel causing DNA fragments to migrate
E) All of the choices are correct.

Accepted Answer

All of the choices correctly describe aspects of gel electrophoresis. A) Larger fragments move more slowly through the gel matrix, B) DNA's negative charge causes it to move towards the positive pole, C) DNA fragments are stained to be visible, and D) an electric current causes DNA fragments to migrate through the gel.