In Reverse Genetics, What is the Correct Order in Which

Question

In reverse genetics, what is the correct order in which the experimenter proceeds?
A) selection of mutants with a phenotype of interest and then mapping to locate the effected gene(s)
B) identification of a gene of interest followed by screening for mutations in that gene and studies of the phenotypes caused those mutations.
C) random bombardment of the DNA with a known mutagen, followed by observation of offspring for newly acquired traits
D) screening individuals by PCR/DNA sequencing to associate altered genes with the trait of interest
E) silencing the genes in question using RNAi, followed by mapping to locate the gene

Accepted Answer

Reverse genetics starts with a gene of interest and then investigates the function of that gene by analyzing the phenotypes resulting from specific mutations in the gene.