You have the ability to measure the transport rate, Q, across a membrane as a function of the concentration of transported solute on the source side, S. How would you experimentally determine the Km for transport?
A) It is the solute con centration at one-h alf the m ximum transport rate B) It is the X -intercept on a plot of 1 /Q against 1 / S C) It is the Y- interc ept o n a p lot of 1/Q against 1/ S D) It is the solute concentration when Q = Q max E) It is -R times the slope of the plot of ln Q vs 1/T