Which of These is Most Important to ChIP (Chromatin Immunoprecipitation)technology

Question

Which of these is most important to ChIP (chromatin immunoprecipitation)technology?
A) an antibody that recognizes specific chromatin structures
B) an antibody that recognizes a nucleosome
C) an antibody that recognizes a transcription factor of interest
D) an antibody that recognizes the DNA cis-element of interest
E) an antibody that recognizes the promoter of interest

Accepted Answer

ChIP technology relies on immunoprecipitation of a specific transcription factor of interest. Antibodies that recognize other chromatin structures (A and B) or DNA elements such as cis-elements or promoters (D and E) may be useful in other assays but are not specific to ChIP.