PCR Cannot Be Successfully Performed Without

Question

PCR cannot be successfully performed without
A) at least 100 starting DNA molecules.
B) at least some sequence information about the region to be amplified.
C) a cDNA version of the region to be amplified.
D) a section of at least 100 kb to amplify.
E) an undamaged,nondegraded DNA sample.

Accepted Answer

PCR requires some sequence information about the region to be amplified in order to design primers that will anneal to the DNA template and initiate amplification. While the other options may affect the efficiency or success of PCR, they are not strictly necessary for the process to occur.