In Microsatellite Analysis,all of the Following are Applicable Except

Question

In microsatellite analysis,all of the following are applicable except
A) must know the sequence of flanking markers.
B) must know the sequence of the repeat units in the microsatellite.
C) polymorphism is a matter of DNA size.
D) electrophoresis.
E) PCR.

Accepted Answer

Microsatellite analysis does not require knowing the sequence of the repeat units in the microsatellite. It primarily relies on the knowledge of the sequence of flanking markers to design primers for PCR, which amplifies the region of interest. The polymorphism detected is due to variations in the length of the DNA segment between the flanking regions, which is analyzed through electrophoresis.