The Most Common Method for Genotyping Allelic SSRs is By

Question

The most common method for genotyping allelic SSRs is by
A) Southern blot analysis
B) FISH protocol
C) PCR and gel electrophoresis
D) sequencing
E) gas chromatography

Accepted Answer

PCR and gel electrophoresis is commonly used to genotype allelic SSRs. PCR amplifies the SSR region and gel electrophoresis separates the amplified fragments based on their size or number of repeats, allowing for identification of different alleles. Southern blot analysis and FISH protocols are less commonly used for genotyping SSRs, while sequencing and gas chromatography are more commonly used for genotyping other types of genetic markers.