Which of the Following is Required in Both Pyrosequencing and Sanger

Question

Which of the following is required in both pyrosequencing and Sanger sequencing?
A) emulsion PCR
B) coated beads
C) DNA polymerase
D) luciferase
E) adapter sequences

Accepted Answer

Both pyrosequencing and Sanger sequencing require DNA polymerase to amplify the DNA sequences being sequenced. Emulsion PCR, coated beads, luciferase, and adapter sequences are all specific to pyrosequencing and not required for Sanger sequencing.