Where Should Barcodes Be Placed in a Gene Deletion Cassette

Question

Where should barcodes be placed in a gene deletion cassette?
A) flanking the selectable marker
B) flanking one of the homologous fragments
C) flanking both homologous fragments
D) in the middle of the selectable marker
E) in the middle of the homologous fragment

Accepted Answer

Barcodes should be placed flanking the selectable marker to ensure they are included in the deletion cassette and can be used for tracking or identification purposes.