What is one major limitation of two -dimensional gel electrophoresis (2DGE)?

A) When products are separated, they tend to leach out of the gel matrix.
B) Only the most abundant products are detected.
C) 2DGE can be run only on nucleic acids.
D) It is extremely costly to execute in a typical molecular biology laboratory.
E) 2DGE is useful only in separating eukaryotic gene products.