During PCR, Heating of DNA is Required To

Question

During PCR, heating of DNA is required to
A) promote attachment of nucleotides to the template.
B) allow DNA ligases to bind fragments together.
C) unwind double-stranded DNA.
D) attach the primer to the template DNA.
E) attach a stop codon.

Accepted Answer

Heating during PCR is used to denature the double-stranded DNA, causing it to unwind into single strands so that primers can anneal and DNA polymerase can synthesize new DNA strands.