How Can Transgenic Organisms Be Used to Identify Transcriptional Enhancers

Question

How can transgenic organisms be used to identify transcriptional enhancers?
A)A reporter gene can be constructed so that its expression is controlled by an enhancer that may potentially exist within a fragment of genomic DNA.
B)A reporter gene can be expressed in every tissue.
C)A gene that is normally expressed in the organism can be cloned under the control of different regulatory elements so that it is expressed in all tissues.
D)A transgene can be integrated in a site that disrupts a gene to determine whether that gene is essential for the cell or organism.

Accepted Answer

A reporter gene can be used to identify transcriptional enhancers by constructing it in such a way that its expression is controlled by a potential enhancer within a genomic DNA fragment. This allows researchers to determine if the enhancer can activate transcription of the reporter gene in the context of the organism's genome.