What is One Major Limitation of Two-Dimensional Gel Electrophoresis (2-DGE)

Question

What is one major limitation of two-dimensional gel electrophoresis (2-DGE)?
A) It is extremely costly to execute in a typical molecular biology laboratory.
B) When products are separated, they tend to leach out of the gel matrix.
C) Only the most abundant products are detected.
D) 2-DGE can be run only on nucleic acids.
E) 2-DGE is useful only in separating eukaryotic gene products.

Accepted Answer

One major limitation of two-dimensional gel electrophoresis (2-DGE) is that it can detect only the most abundant products. Less abundant proteins may not show up on the gel, making it difficult to analyze the entire proteome of a sample.