Question 15

(Multiple Choice)

Place the following steps for cloning DNA in the correct order:
1 - use vector to deliver new rDNA to bacterial or other cells
2 - use restriction enzymes to cut a plasmid and add the desired gene
3 - isolate and cut out a desired gene using restriction enzymes
4 - use DNA ligase to seal the new gene
5 - allow bacterial cells to replicate and produce desired product


A)1,2,3,4,5
B)5,3,2,4,1
C)2,5,3,1,4
D)3,2,4,1,5

Answer