Agarose Gel Electrophoresis Separates Nucleic Acid Fragments According To

Question

Agarose gel electrophoresis separates nucleic acid fragments according to
A) density.
B) shape.
C) size.
D) nucleobase sequence.
E) type of 5C sugar.

Accepted Answer

Agarose gel electrophoresis separates nucleic acid fragments based on their size. The smaller fragments move faster through the gel matrix and are thus further down the gel, while larger fragments move slower and are closer to the top. Density, shape, nucleobase sequence, and type of 5C sugar do not influence fragment separation in agarose gel electrophoresis.