One Way to Detect Alternative Splicing of Transcripts from a Given

Question

One way to detect alternative splicing of transcripts from a given gene is to
A) compare the sequences of different mRNAs made from this gene.
B) compare the sequences of different primary transcripts made from this gene.
C) compare the DNA sequence of this gene to that of a gene known to be constitutively spliced.
D) measure the relative rates of transcription of this gene compared to that of a gene known to be constitutively spliced.

Accepted Answer

Alternative splicing produces multiple mRNA isoforms from a single gene. By comparing the sequences of different mRNA transcripts made from the same gene, one can detect differences in splicing patterns and identify alternatively spliced isoforms. Option B is incorrect because primary transcripts contain introns and are processed into mature mRNA through splicing. Option C is incorrect because comparing the DNA sequence does not directly reveal information about splicing patterns. Option D is incorrect because the rate of transcription does not necessarily correspond to alternative splicing.