RFLPs Can Be Used to Identify Point Mutations Through Which

Question

RFLPs can be used to identify point mutations through which of the following mechanisms?
A)The mutation can occur outside of a restriction site causing a change in the cutting pattern for restriction enzymes.
B)The mutation can occur causing a shortening of the mRNA that is then compared to wild type.
C)The mutation can occur in a restriction site,causing a change in the cutting pattern for restriction enzymes.
D)The mutation can be silent in the coding for protein resulting in a different amino acid being inserted.

Accepted Answer

RFLPs are used to detect variations in DNA sequences and can identify point mutations if the mutation occurs in a restriction enzyme site, causing a change in the cutting pattern of the enzyme. The other choices do not directly relate to the use of RFLPs in identifying point mutations.