Which of the Following is the Correct Order of Steps

Question

Which of the following is the correct order of steps for the CRISPR- Cas mechanism?
A)spacer acquisition,biogenesis,target interference
B)target interference,transcription,biogenesis
C)cleavage,target interference,biogenesis
D)transcription,cleavage,spacer acquisition

Accepted Answer

The CRISPR-Cas mechanism follows the following steps in order: Spacer acquisition (acquisition of foreign genetic material), Biogenesis (processing of CRISPR RNA and Cas proteins), and Target interference (binding of CRISPR RNA and Cas proteins to foreign DNA and cleavage of the DNA). Therefore, option A is the correct order of steps.