What is the Most Sensitive and Rapid Primary Isolation Liquid

Question

What is the most sensitive and rapid primary isolation liquid media for Mycobacterium spp.?
A) Thioglycollate
B) Cooked meat
C) 5% NaCl broth
D) Middlebrook 7H12

Accepted Answer

Middlebrook 7H12 is the most sensitive and rapid primary isolation liquid media for Mycobacterium spp. It contains a high concentration of nutrients, including glucose, which supports the growth of slow-growing mycobacteria. Thioglycollate and cooked meat are not commonly used for the isolation of Mycobacterium spp. 5% NaCl broth is used for the isolation of halophilic bacteria.