When Making Slides of Thin Materials Such as Urine and Cerebrospinal

Question

When making slides of thin materials such as urine and cerebrospinal fluid (CSF)
A) spread the material once, let the specimen dry, then spread another layer on top to ensure enough specimen to stain.
B) use the two-slide pull method to ensure evenly distributed material over the slides.
C) dip a swab into the specimen, then roll the swab over the glass slide to ensure adequate coverage for staining.
D) mark the area of the sample drop on the reverse side with a wax pencil and then place a drop of the specimen into the marked area.

Accepted Answer

Marking the area of the sample drop on the reverse side with a wax pencil and then placing a drop of the specimen into the marked area ensures that the specimen is accurately placed and does not spread too thin or too thick. This method also allows for easier tracking of the location of the specimen on the slide during staining and observation.