Which DNA Sequence Would Be the Most Difficult to Assemble

Question

Which DNA sequence would be the most difficult to assemble?
A) A sequence with an ORF
B) A sequence consisting of several short repeated fragments
C) A sequence with roughly equal proportions of As,Cs,Gs,and Ts
D) A sequence with a promoter
E) A sequence that contains the fragment CAGCTATT

Accepted Answer

A sequence consisting of several short repeated fragments would be the most difficult to assemble because the repeated fragments can cause confusion and errors in the assembly process. It may not be clear which fragment belongs where, leading to difficulties in determining the correct order of the fragments.