What is the major difference between the DNA sequencing techniques developed by Sanger and others in the 1970s and high-throughput sequencing methods?

A) High-throughput sequencing enables the direct sequencing of a chromosome from one end to the next.
B) Unlike the earlier technology,high-throughput sequencing involves cutting the DNA into fragments.
C) Unlike the earlier technology,high-throughput sequencing relies heavily on miniaturization techniques.
D) High-throughput sequencing involves chemical modification of nucleotides,whereas the earlier technology does not.
E) Unlike the earlier technology,high-throughput sequencing does not use PCR.