Liquid Media Containing Heat-Sensitive Components Would Best Be Sterilized By

Question

Liquid media containing heat-sensitive components would best be sterilized by
A)Ultraviolet (UV) light at 500 nm.
B)freezing.
C)lyophilization.
D)membrane filtration.

Accepted Answer

Liquid media containing heat-sensitive components such as enzymes or antibodies can be sterilized by membrane filtration, which involves the passage of the liquid through a filter with pores small enough to trap microorganisms but allow the liquid to pass through. This method is effective for sterile filtration of heat-sensitive solutions that cannot be autoclaved or subjected to other high-temperature sterilization methods. Ultraviolet (UV) light, freezing, and lyophilization are not effective sterilization methods for liquid media containing heat-sensitive components.