An experiment was designed to obtain nonspecific transcription from both strands of a DNA molecule.Which of the following strategies would be most effective in achieving this?

A)Include the RNA holoenzyme in the reaction.
B)Use the core enzyme of RNA polymerase.
C)Enrich the preparation with sigma subunit.
D)Use intact DNA .
E)Include the RNA holoenzyme in the reaction and use the core enzyme of RNA polymerase are both effective.