Which of the Following is Not Required for Mammalian Cell

Question

Which of the following is not required for mammalian cell polyadenylation of pre-mRNA?
A)PAP
B)CPSF
C)PABII
D)RNA polymerase I
E)RNA polymerase II

Accepted Answer

RNA polymerase I is not involved in mammalian cell polyadenylation of pre-mRNA. RNA polymerase II is responsible for transcribing pre-mRNA, while PAP, CPSF, and PABII are all necessary factors for adding the poly(A) tail to the 3' end of the pre-mRNA transcript.