Which of the Following Processes Allows Scientists to Identify the 5'-Ends

Question

Which of the following processes allows scientists to identify the 5'-ends of transcripts?
A)SAGE
B)CAGE
C)RNAi analysis
D)oligonucleotide array
E)PCR

Accepted Answer

CAGE (Cap Analysis of Gene Expression) is a method used to identify the 5’-end caps of transcripts, allowing for accurate mapping of transcription start sites. SAGE (Serial Analysis of Gene Expression) is a method used to quantify gene expression, but it does not specifically identify the 5’-ends of transcripts. RNAi analysis and oligonucleotide arrays are methods used to study gene function and expression, but do not specifically identify transcription start sites. PCR (Polymerase Chain Reaction) can amplify specific DNA sequences, but it does not specifically identify the 5’-ends of RNA transcripts.